Transcriptional profiling of Pseudomonas aeruginosa WT and Pho mutant colony biofilms in response to ethanol and Candida albicans. Transcriptional profiling of Pseudomonas aeruginosa WT and Pho mutant colony biofilms in response to ethanol and Candida albicans

Pseudomonas aeruginosa is an opportunistic pathogen frequently isolated from co-infections with Candida albicans and other ethanol-producing organisms. We found that ethanol, including that produced by C. albicans, stimulates the PhoB regulon in P. aeruginosa. We identify a subset of PhoB-regulated genes as differentially expressed in response to ethanol which are slightly different than the set of genes with increased expression in a genetically induced low phosphate response via pstB mutation. Overall design: Genome-wide transcriptional profiles of Pseduomonas aerguinosa after growth for 24 hours in colony biofilms on minimal media with or without 1 % ethanol or in co-culture with ethanol-producing (adh1∆/ADH1) or non-ethanol-producing (adh1∆/∆) Candida albicans.