Data for the article entitled: Multiphoton microscopy at a microwatt level via gain-managed nonlinear amplification and pulse picking

The following files include data presented in the manuscript “Multiphoton microscopy at a microwatt level via gain-managed nonlinear amplification and pulse picking,” written by Katarzyna Kunio, Grzegorz Soboń, and Jakub Bogusławski.Fig. 2a – Characterization of the pulse: optical spectrum at the output of the oscillator and at the output of the bandpass filterFig. 2b – Characterization of the pulse: autocorrelation with the Gaussian fit at the output of the oscillatorFig. 2c – Characterization of the pulse: autocorrelation with the Gaussian fit at the output of the bandpass filterFig. 3a – Characterization of the pulse after amplification and compression at different values of the pump power: optical spectraFig. 3b – Characterization of the pulse after amplification and compression at different values of the pump power: FROG-retrieved temporal intensity with temporal phaseFig. 4a – Characterization of the pulse at the pump power of 2.26 W: optical spectrumFig. 4b – Characterization of the pulse at the pump power of 2.26 W: FROG-retrieved temporal intensity with temporal phaseFig. 4b (inset) – FROG spectrograms: measured and retrievedFig. 5a – Analysis of the influence of lowering both the repetition frequency and average power at the sample on TPE microscopy: (a) TPE fluorescence images of convallaria majalis obtained by gradually decreasing the repetition frequency while simultaneously decreasing the average power at the sample. Scale bars: 80 µm.Fig. 5b – Change in the SNR depending on the repetition frequency as a function of number of averaged framesFig. 6 – SHG microscopy images of urea microcrystals obtained by gradually decreasing the repetition frequency while simultaneously decreasing the average power at the sample. Scale bars: 80 µm. Fig. 7a – Comparison of pixel intensities in the images obtained at the same values of the mean signal and different values of the repetition frequency measured in row 250 of images depicting convallaria majalis. Scale bars: 80 µmFig. 7b – Comparison of pixel intensities in the images obtained at the same values of the mean signal and different values of the repetition frequency measured in row 110 of images depicting urea microcrystals. Scale bars: 80 µmFig. 7c – Oscilloscope traces of the detector response measured while imaging urea microcrystals at different repetition frequenciesFig. 8a – Spectral phasor-based 3PEF imaging of epidermal cells without staining: transmission spectra of sine and cosine filtersFig. 8b – Spectral phasor-based 3PEF imaging of epidermal cells without staining: epidermal cell images obtained without an additional filter in front of the PMT (INT), with cosine (COS), and with sine (SIN) filters. Scale bars: 200 µm.Fig. 8c – Spectral phasor-based 3PEF imaging of epidermal cells without staining: spectral phasor plotFig. 8d – Spectral phasor-based 3PEF imaging of epidermal cells without staining: spectral phasor-based color-coded image of epidermal cells. Scale bars: 200 µm.