Paralichthys olivaceus Genome sequencing

To our knowledge, there is only one other report of a 2nd generation of clonal colony of fish (Oreochromis niloticus) by Müller-Belecke and Hörstgen-Schwark, who studied the survival rate, reproductive traits, and mean body weight of a 2nd generation clones of tilapia5. A clonal line of Japanese flounder was established for the first time in China {Anonymous:2013us}. Because all individuals of the clonal line are females, it is impossible to produce 2nd generation clones by male and female mating. Here, we describe the creation of a 2nd generation clonal group of Japanese flounder by induced meiogynogenesis using eggs spawned by 1st generation clones