AlkB-facilitated RNA methylation sequencing (ARM-seq) and Demethylase-thermostable group II intron RT tRNA sequencing (DM-tRNA-seq) [Proximal Colon]

The goal of this study was to determine the state of the tRNA transcriptome in Proximal Colon tissue of Mus Musculus (Mouse). This includes expression data of mature tRNAs and tRNA fragments (tRFs), as well as associated modification states of these RNAs assessed using mismatching percentages from sequencing data. Overall design: Included are ARM-seq and DM-tRNA-seq libraries derived from isolated total small RNA from C57BL/6J male Proximal Colons. These experiments were performed in triplicate (biological) and subjected to NGS.