Single-cell chromatin and transcriptome dynamics of Synovial Fibroblasts transitioning from homeostasis to pathology in TNF-driven arthritis

We undertook an integrative approach by combining sc transcriptomic (scRNA-seq) and chromatin accessibility data (scATAC-seq) to uncouple and characterize the homeostatic and pathological functions of SFs in the human TNF overexpressing (hTNFtg) mouse model. Our study defines the underlying molecular switches that determine the staging and progression of TNF-mediated disease from healthy to early inflammatory and subsequent destructive synovial tissue