Kinetics of the exchange of GpA TM peptides between APol aggregates.

Donor- and acceptor-labelled GpA TM (each 0.25 µM) domains were separately solubilized in 20 µM APol at a final polymer/peptide ratio of 40∶1. (A) After incubation at 37°C, donor and acceptor were mixed, and emission spectra (normalized at 525 nm) were recorded every 10 min over a time period of 17 h at 25°C. (B) The energy transfer increased over hours to the level determined by steady-state FRET measurements due to mixing of donor- and acceptor-labelled peptides. (C) Energy transfer change due to peptide exchange in 5 mM DDM micelles was completed after some dozen minutes.