Gene expression data of glioblastoma multiforme U-87 cells exposed to targeted electromagnetic fields. Gene expression data of glioblastoma multiforme U-87 cells exposed to targeted electromagnetic fields

A novel application of a non-invasive, electromagnetic field technology has a significant inhibitory effect on the proliferation of glioblastoma multiforme U-87 MG cells in culture. This study reports the cellular and molecular responses of U-87 MG cells to the effects of a tunable, non-ionizing radiation technology that does not induce the serious side effects commonly observed with chemotherapy. The broadband (RF/Low Microwave) electromagnetic field is tuned by means of oscillating wave forms, selected reference materials and a positive feedback loop (RGFIELDS™). By simultaneously targeting specific molecules (oligonucleotides and proteins) that contribute to pathogenesis of glioblastoma with this technology, the continuous exposure of cells for 54 h results in the inhibition of cell growth and a concurrent increase in cell death. We used microarrays to elucidate the cellular processes involved in the response of U-87 MG cells to exposure to RGFIELDS™ and identified mRNA and non-coding RNA sequences that are differentially modulated 1.5 fold or more. Overall design: Total RNA was isolated from three independent biological replicates of U-87 MG cells exposed continuously for 54 h to RGFIELDS™ targeting selected reference materials (oligonucleotides and proteins), and from unexposed control cells cultured under identical conditions.