Genome-wide analysis of m6A modification

N6-methyladenosine (m6A) might exert stronger affection on circRNA expression, beyond linear genes.We confirmed the view by analysing total RNA meRIP-Seq from GSE114150 and three other sets of data using RNA sequencing. Here, we study the difference in circRNA levels between wild (WT) and experimental (D395A-mutation; METTL3-knock-out) groups when m6A level changes. Results reveal knockout and D395A mutation of METTL3 could both increase the circRNA expression and the ratio of circular-to-linear. Our data propose a new pattern of circRNAs development and expand the understanding of m6A functions. Overall design: mettl3 is knockedout using Crispr in embryonic stem cell in order to study the interaction between m(6)A and circRNA.