Cellular resolution data from wild-type and reconstituted eve stripe 2 enhancer reporter constructs

Data accompanying Vincent et al. 2016. Files include the following:
pointclouds_costain.zip : Cellular resolution data from individual embryos containing reporter constructs for wild-type and reconstituted versions of the enhancer controlling the second stripe of the even-skipped expression pattern (eve2). Embryos were stained for fushi tarasu (ftz) in the coumarin channel and LacZ in the Cy3 channel. A huckebein (hkb) co-stain is also included in the Cy3 channel to normalize expression levels between lines (see Wunderlich, et al. 2014). Each Pointcloud file contains the xyz coordinates, ftz expression level, and LacZ/hkb expression level for every nucleus in the embryo. All embryos were stained with the same probes in the same batch.
atlas_costain.vpc : A cellular resolution gene expression atlas of an average Drosophila melanogaster embryo. Contains expression patterns generated by wild-type and reconstituted versions of eve2. Computational methods for atlas generation can be found in Fowlkes, et al. 2008. Pointclouds used to make the atlas are included in pointclouds_costain.zip. Cellular resolution data for eve2 regulators can be downloaded from the Berkeley Drosophila Transcription Network Project (http://bdtnp.lbl.gov/Fly-Net/bidatlas.jsp).
pointclouds_nocostain.zip : Cellular resolution data from individual embryos containing reporter constructs for wild-type and reconstituted versions of eve2. These embryos were not co-stained for hkb. 
atlas_costain.vpc : A cellular resolution gene expression atlas of an average Drosophila melanogaster embryo. Contains expression patterns generated by wild-type and reconstituted versions of eve2. This atlas was generated from embryos that were not co-stained for hkb.