RNA sequencing of human regulatory T cell clones, both Faecalibacterium prausnitzii-reactive DP8a (CD4,CD8alphalow) Tregs and (CD4,CD25high,CD127low) Foxp3 Tregs obtained from the colon lamina propria and from the blood.

We generated human DP8a Treg (CD4, CD8alow, CCR6+, CXCR6+, F. praunsitzii-reactive) and FoxP3 Treg (CD4, CD25high, CD127low) clones from healthy colon lamina propria-derived lymphocytes (LPL) (from colorectal cancer patients) and from blood lymphocytes from healthy volunteers). Through DEseq2 analysis) we identified activation-induced genes in these clones and compared gene expression between resting and activated DP8a and Foxp3 clones. Overall design: This experiment compare the gene expression of 8 groups of human regulatory T cell clones: resting and activated colon-derived DP8a clones, resting and activated colon-derived FoxP3 Treg clones, resting and activated blood-derived DP8a clones and resting and activated blood-derived FoxP3 clones