Self-organizing Single-Rosette Brain Organoids (SOSRS) from Human Pluripotent Stem Cells

We report single-cell RNA-sequencing data characterizing the cell types and variability in self-organizing single rosette forebrain organoids differentiated for 1mo, 3mo, and 5mo with 4, 6, and 3 SOSRS used, respectively. The data shows increasing cellular diversity over differentiation time with a high degree of similarity in the cell diversity between SOSRS differentiated to the same timepoint. Overall design: scRNA-sequencing for brain organoids at 1, 3, and 5 months with 4, 6, and 3 SOSRS used to compare cell types and variailbility. Cells from each organoid were labelled with an antibody-based cell hashing technique to allow multi-sample pooled analyses to reduce batch effects.