Number of qPCR traces that amplify for all WT samples prior to melt curve analysis or sequencing.
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Eg. qPCR specificity prior to sequencing. qPCR provides quantitative copy number data and positives are orthogonally confirmed using sanger sequencing. This helps to filter non-specific qPCR signals and/or confirms which nucleotide is mutant.
创建时间:
2019-10-03



