Transcriptomic analysis of B cells responding to Toxoplasma gondii infection.

Table of genes included (threshold of > = 5 CPM in any sample) in differential gene expression analysis. For each gene the Ensembl ID, MGI ID, gene symbol and descriptions are listed. The CPM from 3 samples of peritoneal B-1a, B-1b, and B-2 at naïve (N), chronic (Ch), and day 5 (D5) of secondary infection in both A/J (AJ) and C57BL/6J (B6) mice are given. The average CPM was calculated for each set of samples and average counts less than 5 CPM were raised to 5 to reduce the effect of low expression genes in further analysis. The log2 fold change (FC) of gene expression was calculated using the average CPM for each cell type (B-1a, B-1b, and B-2) of each strain (A/J and C57BL/6J) as they progressed from naïve to chronic infection or chronic to D5 secondary infection. The Log2 FC was also calculated from naïve to D5 of secondary infection. The Log2 FC between A/J and C57BL/6J was calculated for each cell type and time point. Genes were then ranked by the number of comparisons that exhibited greater than 4FC by the indicated comparison. (XLSX)