MG36/37: Grape FS shoot-tip Endophytic Microbiome-Whole metagenome-Bacteria Raw sequence reads. endophyte metagenome

This study was undertaken to assess the endophytic bacterial diversity prevailing in the shoot-tip tissue of grape cv. Flame Seedless through whole metagenome profiling (Sample ID: MG36/37). DNA isolated from the surface sterilized tender shoot-tip tissue using MO BIO Powerfood microbial DNA isolation kit was subjected to whole metagenome profiling with Illumina Hiseq platform with 2x250 bp. The derived raw reads (12.70 million) after adapter removal and filtration steps showed 86,347 contigs (de novo metagenome assembly). Phylogenetic analysis using MEGAN software showed predominantly Streptophyta under Eukaryota. Data analysis was carried out after filtering the contigs corresponding to Viridiplantae showed 88 ORFs with Proteobacterial predominance followed by Actinobacteria. Further downstream phylogenetic analysis showed 28.6% share as Ralstonia solanacearum (Betaproteobacteria), which is a common pathogen affecting solanaceaous crops, but not of grape. Hence the phylogenetic and functional diversity analyses were undertaken excluding the 35 contigs that corresponded to R. solanacearum.