DataSheet_1_Leukocyte cell population data in patients with cardiac surgery and cardiopulmonary bypass: A potential readily available tool to monitor immunity.docx

PurposeCardiac surgery with cardiopulmonary bypass triggers sterile inflammation that is responsible for post-operative morbidity. Automated flow cytometry devices used for leucocyte count provide cell population data (CPD) regarding fluorescence intensity, size and granularity of leukocytes that have never been studied in the context of sterile inflammation. Our objective was to explore leukocyte cell population data in patients undergoing cardiac surgery with cardiopulmonary bypass in order to determine whether CPD could be used to monitor immune cell activation.MethodsThis is an ancillary study of a cohort of patients undergoing cardiac surgery with cardiopulmonary bypass. Cell population data (CPD) extracted from a routine automated flow cytometer were analyzed (Fluorescence targeted to nucleic acids). The time points of interest were: pre-operative, postoperative and 5 days after surgery. The variations in those parameters were studied. Data were then compared between patients according to the occurrence of a composite criteria (supra-ventricular arrythmia, stroke, acute renal failure, and/or death).ResultsData from 1453 patients were analyzed. The neutrophil count, fluorescence granularity (NE-SCC), intensity (NE-SFL) and size (NE-FSC) increased with surgery. Heterogeneity of neutrophils decreased in terms of fluorescence granularity (NE-WX) and size (NE-WZ) but increased in terms of intensity (NE-WY). The lymphocyte count decreased with surgery. While fluorescence granularity (LY-X) and size increased (LY-Z), Lymphocyte intensity decreased (LY-Y). Lymphocytes were less heterogeneous in terms of their granularity, size and intensity after surgery (LY-WX, LY-WY, LY-WZ). Patients who developed the composite complication criteria had a higher pre-operative neutrophil count (5.08 [3.89;6.95] vs 4.76 [3.60;6.13], p = 0.02; AUC = 0.56 [0.51;0.60]), and more heterogeneous neutrophils in terms of fluorescence granularity (NE-WX, AUC = 0.57 [0.52;0.62]) and intensity (NE-WY, AUC 0.61 [0.56;0.65]). Those patients also had lower pre-operative lymphocyte count (1.49 [1.10;1.14] vs 1.81 [1.39;2.39], p

目的：心脏手术联合心肺旁路术可诱发无菌性炎症，该炎症是术后发病率的主要原因。自动流式细胞计数仪在白细胞计数中的应用，提供了关于从未在无菌性炎症背景下研究过的白细胞群体数据的细胞群体数据（CPD），包括荧光强度、大小和颗粒度。本研究旨在探讨进行心脏手术联合心肺旁路术患者的白细胞群体数据，以确定细胞群体数据（CPD）是否可用于监测免疫细胞活化。方法：本研究为对接受心脏手术联合心肺旁路术患者队列的辅助研究。从常规自动流式细胞计数仪中提取的细胞群体数据（CPD）被进行分析（荧光靶向核酸）。感兴趣的时点包括手术前、手术后及术后5天。对这些参数的变化进行了研究。然后，根据复合标准（室上性心律失常、中风、急性肾衰竭和/或死亡）的发生情况，对患者进行了数据比较。结果：分析了1453名患者的数据。中性粒细胞计数、荧光颗粒度（NE-SCC）、强度（NE-SFL）和大小（NE-FSC）随手术增加。中性粒细胞的异质性在荧光颗粒度（NE-WX）和大小（NE-WZ）方面减少，但在强度（NE-WY）方面增加。淋巴细胞计数随手术减少。荧光颗粒度（LY-X）和大小增加（LY-Z），淋巴细胞强度降低（LY-Y）。手术后，淋巴细胞在其颗粒度、大小和强度方面的异质性降低（LY-WX、LY-WY、LY-WZ）。发生复合并发症标准的患者，术前中性粒细胞计数较高（5.08 [3.89;6.95] vs 4.76 [3.60;6.13]，p = 0.02；AUC = 0.56 [0.51;0.60]），并且中性粒细胞的荧光颗粒度（NE-WX，AUC = 0.57 [0.52;0.62]）和强度（NE-WY，AUC 0.61 [0.56;0.65]）的异质性更高。这些患者术前淋巴细胞计数也较低（1.49 [1.10;1.14] vs 1.81 [1.39;2.39]，p