RNA sequencing of HUVECs stimulated with VEGF, OSM+VEGF, or CNTF+CNTFR+VEGF with or without STAT3 knock-down

The role of STAT3 signaling induced by stimulation of IL-6 cytokine family members such as OSM and CNTF on the angiogenic response of endothelial cells is still not fully understood. To assess the impact of this pathway on angiogenesis, this study compares human umbilical vein endothelial cells (HUVECs) with or without STAT3 knock-down introduced by siRNA and stimulation with VEGF, OSM+VEGF and CNTF+VEGF in the presence of the CNTF receptor (CNTFR). Overall design: mRNA profiles of HUVECs stimulated with VEGF (n=4), OSM+VEGF (n=4), or CNTF+CNTFR+VEGF (n=4) for 24h transfected with control siRNA. The samples were compared to HUVECs transfected with STAT3 siRNA and incubated by the same cytokines for 24 (n=4 each).