Tm of hHsp90 CTD wild type and alanine mutants.
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[a]The detected fluorescence signal corresponds to the denaturation state of hHsp90. The melting temperature Tm of hHsp90 CTD wild type, cold spot, and alanine mutants was determined from the derivative of the fluorescence data by the implemented software (qPCRsoft V2.0.37.0, Analytik Jena AG, Germany). The mean value and standard deviation were calculated from four independent measurements in reaction buffer with 100 mM Tris at pH 7.5 in °C.[b]Difference in the Tm with respect to the wild type in °C.
创建时间：
2015-12-02
