five

Mapping the chicken chromatin accessibility landscape by UUATAC-seq

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NIAID Data Ecosystem2026-05-02 收录
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https://www.ncbi.nlm.nih.gov/sra/SRP479742
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The current understanding of regulatory sequences in the vertebrate genome is still incomplete. By introducing an adapter switching strategy, we developed an ultra-throughput ultra-sensitivity single-nucleus ATAC-seq (UUATAC-seq) protocol that enables construction of high-quality chromatin accessibility landscape from one species in a one-day experiment. By using UUATAC-seq, we mapped open chromatin regions for five representative vertebrate species at the landscape scale. Overall design: UUATAC-seq was performed across 18 tissues from one female chicken to profile over 200,000 nuclei.
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2025-08-27
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