Protein constructs obtained from the different expression vectors.

Sequences are presented using one-letter code for amino acids. attB1: amino acid sequence encoded by the attB1 cloning site corresponding to TSLYKKAGS when the entry clone was prepared though BP cloning and TSLYKKAGSAAAPFT when the entry clone was prepared through TOPO recombination (NapC, P450, LPR1, PHF, PHT1;4, ceQOHR, AtHMA1, Bcl-xL). MP: amino acid sequence of the different membrane proteins. Mistic: amino acid sequence of the fusion tag Mistic. Strep: amino acid sequence of the Strep-tag II fusion tag corresponding to WSHPQFEK.*The Strep-tag II was fused to the C-terminus of most proteins, except for proteins AtHMA3, AtHMA4 and Bcl-xL for which the Strep-tag II was located at the N-terminus of the MP sequence.