Analytical characteristics of bisulfite and methylation specific real-time, quantitative PCR.
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a)bisulfite-treated B19V insert excised from pB19-M20.b)amplification of residual, unmethylated target following in vitro M.SssI treatment.Unmethylated or in vitro methylated, bisulfite-treated B19V inserts from pB19-M20 plasmid have been quantified spectrophotometrically and 10-fold diluted to obtain quantitative standards for the definition of calibration curves. Analytical characteristics have been determined from triplicate amplification of standards in the range 102–108 target copies/reaction. For calibration curves, R2 is >0.99 and CV is 0.16–0.24.
创建时间：
2015-12-02
