Table 1 in The complex case of the calcareous sponge Leucosolenia complicata % Porifera: Calcarea): hidden diversity in Boreal and Arctic regions with description of a new species

Table 1. Amplification and sequencing primers and PCR conditions
MarkerPrimersPCR conditionsReference28S rRNA28S-C2F5 min—94°C* 35 × [1 min—95°C*ChombardGAA AAG AAC TTT GRA RAG AGA GT45s—50°C* 1 min—72°C]* 7 min—72° Cet al. 199828S-D2RTCC GTG TTT CAA GAC GGG18S rRNA18S-328F5 min—94°C* 35 × [1 min—94°C* 1Alvizu et al.CCTGGTGATCCTGCCAGmin—50°C* 2 min—72°C]* 7 min—72°C201818S-HI + RCAACTAAGAACGGCCATGCAC18S-329RTAA TGA TCC TTC CGC AGG TT18S-A-FCAG CMG CCG CGG TAA TWCHistone H3Por_h3f5 min—94°C* 35 × [15 s—95°C*This studyATG GCC CGT ACC AAG CAG ACT GC30s—50°C* 45 s—72°C]* 7 min—72°CPor_h3rATA TCC TTG GGC ATG ATG GTG AC