Glycoproteomic analysis of Haloferax volcanii strains H53, DaglB, and Dagl15

An in-depth glycoproteomic analysis of Haloferax volcanii has been performed, comparing the wildtype H53 with knockout mutants of aglB and agl15. While AglB-dependent glycosylation has been described to occur under standard culture conditions, the Agl15-dependent glycosylation pathway has previously been described to be only active under low-salt conditions. In this dataset, the largest archaeal glycoproteome could be identified with more than 40 N-glycoproteins and more than 100 N-glycopptides. It reveals not only that both glycosylation pathways can be active under the same conditions but also that the same glycosites can be modified by different glycans from both glycosylation pathways.