Programmed Death Ligand 1 in Tumor Margins with Implications of Regulation by Interferon Gamma, and a Novel Immuno-Oncologic Transcriptional Signature for Malignant Transformation. Programmed Death Ligand 1 in Tumor Margins with Implications of Regulation by Interferon Gamma, and a Novel Immuno-Oncologic Transcriptional Signature for Malignant Transformation

Basic analysis of 5 pairs of tumor margin and invasive OSCC tumor tissue using basic nsolver analysis showed, PD-L1 and IFN-γ 6 gene signature to be higher in morphologically normal epithelial tumor margin than in dysplastic tumor margin, yet the invasive OSCC area was the highest in PD-L1 and IFN-γ 6 gene signature. It also demosntrates 38 significant Immuno oncologic gene signature for maligant transformation relative to tumor margins. Overall design: Under an Institutional Review Board (IRB) approved protocol and patient consent whole excision tumor tissues were collected prospectively at the University of Maryland School of Medicine (UMSOM) under (HP-00073603), and retrospectively under (IRB HP-00088284).Five tumors were mapped for tumor margins and invasive area of OSCC for RNA extraction using nCounter Human Pan Cancer IO-360 nanoString Technology .