Ovary, Testis and Accessory Gland sequencing of Drosophila species

Purpose: Sequencing of gonads of Drosophila willistoni species for identification of sex biased genes and other purposes.Methods: Virgin flies were collected and aged 6 to10 days before dissecting 2 to 3 replicates of testes or ovaries. Total RNA was extracted using the Arcturus PicoPure kit . Illumina TruSeq RNA library kits were used to poly A select and reverse-transcribe mRNA, shear cDNA into 150 bp fragments, and produce libraries for paired end 70 bp sequencing on a HiSeq2000. Real Time Analysis v1.13 from Illumina module processed images, called bases, and provided base qualities.