RNA-seq for polyphenols treated Pseudomonas syringae

Bacteria use a variety of mechanisms, such as two-component regulatory systems (TCSs), to rapidly sense and respond to distinct conditions and signals in their host organisms. For example, a type III secretion system (T3SS) is the key determinant of the virulence of the model plant pathogen Pseudomonas syringae and contains the TCS RhpRS as a key regulator. However, the signal sensed by RhpRS remains unknown. We found that RhpRS directly senses plant-generated polyphenols and responds by switching off P. syringae T3SS via crosstalk with alternative histidine kinases. Through a chemical screen, we identified three natural polyphenols (tannic acid, 1,2,3,4,6-pentagalloylglucose and epigallocatechin gallate) that induced the expression of the rhpRS operon in a RhpS-dependent manner. Overall design: RNA-seq for polyphenols treated Pseudomonas syringae and microbiota analysis for Arabidopsis thaliana Both 'Psph-wt-MM*' and 'PSPH-WT-MM*' are wild-type Pseudomonas savastanoi pv. phaseolicola 1448A strain that grown in minimal media but belong to different sequencing batches. The *MM* and *mock samples are also the same cases, they are all wild-type stain cultured in minimal media. In the submitted RNA-seq data, Psph-wt-MM is the control group for L13-NAR500-MM; PSPH-WT-mock is the control group for PSPH-WT-EGCG-10; PSPH-WT-MM is the wild type sample for rhpS mutant and rhpS/3550/3736/5115