RKIP regulates bone homeostasis by mediating the differentiation fate of bone niche macrophages.

As central cells involved in osteoimmunology, bone niche macrophages possess diverse functions, and their differentiation fate regulates bone homeostasis. Elucidation of the underlying mechanism involved in macrophage differentiation is important for developing new therapeutic targets for osteoporosis. Our RNA-seq data showed that the differentially expressed genes in BMMs after RKIP knockout were involved in the pathways of cell adhesion and osteoclast differentiation. To investigate RKIP in the regulation of macrophage differentiation, we isolated BMMs from RKIP+/+ and RKIP-/- mice and performed transcriptome sequencing (RNA-seq). We then performed gene expression profiling analysis using data obtained from RNA-seq of 2 different cells.