Purification of mRNA and elimination of dsRNA during in vitro transcription

mRNA is a promising technology for vaccines and gene therapies, but large-scale production remains challenging due to its instability and unwanted byproducts. This study developed a more efficient and scalable mRNA production platform. We introduced a simple precipitation purification method, a negatively charged material that improved yield and reduced double-stranded RNA, and a one-step reaction–purification approach using oligo(dT) media. Together, these methods improve mRNA purity, reduce immunogenicity, and support safer, more cost-effective manufacturing. This platform may help advance the clinical use of mRNA vaccines and therapies across broader medical applications.