Transcriptional Activation of Regenerative Haematopoiesis via Niche Sensing [CB vs mPB scRNA-seq]

Single cell RNA-seq analysis was performed on sorted population of CD45+/CD34+ HSPCs from cord blood (CB) and mobilized peripheral blood (mPB) donors, after co-culture with vascular niche cells, to uncover differential transcriptional output between HSPC derived from CB and mPB origins. Overall design: Human CB and mPB HSPCs were harvested isolated and sorted. HSPCs were introduced into co-culture with vascular niche ECs. Cultures were harvested and CD45+/CD34+ HSPCs were resorted and processed for 10X Chromium scRNA-seq analysis.