Atomic force microscopy indentation data of stiff and compliant polyacrylamide hydrogels

<b><br>Atomic force microscopy indentation data of stiff and compliant polyacrylamide hydrogels</b><br><br>This dataset consists of 110 force-distance curves of two (stiff and compliant) polyacrylamide (PAAm) gels recorded using atomic force microscopy. Each file represents one measurement. The file names indicate whether a measurement was performed on the stiff or on the compliant gel. For each gel, curves were recorded from six regions of interest (ROIs).<br><br>The data were recorded using a JPK NanoWizard 4 and a PNP-TR-TL cantilever (Nanoworld) with a spring constant of 0.08 N/m. For probing, a bead with a radius of 5 µm was attached to the cantilever. The cantilever was calibrated using the thermal noise method in phosphate buffered saline (PBS) at room temperature.<br><br>The hydrogels were produced according to the protocol described in [1]. The gels have an expected Young's modulus of 0.8 to 1.2 kPa (compliant) and 25 to 28 kPa (stiff).<br><br><br><b>References</b><br>[1] G. Rosso et al., Schwann cells and neurite outgrowth from embryonic dorsal root ganglions are highly mechanosensitive, Nanomedicine: Nanotechnology, Biology and Medicine, 2016, doi:10.1016/j.nano.2016.06.011<br><br>