ATP-dependence of microsome-assisted folding.

As a model of in vivo oxidative folding in the ER, microsomes derived from rat liver were used to fold linear peptide. Only the timepoint with the largest difference is shown. Values given are Mean ± Standard Deviation (n = 3).
†No native peptide could be detected in the indicated condition.
*Indicates that this peptide showed a statistically significant difference between microsomes that were ATP-depleted and untreated microsomes (paired, one-tailed t-test of three independent experiments; P < 0.05).