Figure 5

A-B. MRC-5 cells were transduced with lentivirus expressing a control or MyD88 gene and subjected to puromycin selection. Cells were either mock-infected or infected with TB40/E-mCh virus at an MOI of 0.05 and harvested at 7 dpi for RNA isolation and samples were subjected to RT-qPCR analysis using the RT2 profiler PCR array. A. Volcano plot of the genes modulated in uninfected Myd88-transduced cells. The blue box represents genes that were downregulated and red box represents the genes that were upregulated more than 4-fold. B. Volcano plot of the genes modulated in HCMV infected MyD88-transduced cells. The blue box represents genes that were downregulated and red box represents the genes that were upregulated more than 4-fold. C. Graph shows the representative ISGs that remained unchanged during HCMV infection in both conditions and ISGs that were significantly downregulated (red box) or upregulated by more than 4-fold (blue box).