Impact of BCG vaccination on the repertoire of human gamma/delta T cell receptors (ms data)

This data archive contains the raw data from a study of gamma/delta T cell repertoires from participants receiving Bacille-Calmette Guerin (BCG) vaccination. Abstract (as submitted to Frontiers Immunology, 2022-NOV): Tuberculosis (TB) caused by Mycobacterium tuberculosis (Mtb) infection is a serious threat to human health. Vaccination with BCG prevents the development of the most severe forms of TB disease in infants and was recently shown to prevent Mtb infection in previously uninfected adolescents. gdT cells play a major role in host defense at mucosal sites and are known to respond robustly to mycobacterial infection. However, our understanding of the effects of BCG vaccination on gd T cell responses is incomplete. In this study we performed gd T cell receptor (TCR) repertoire sequencing of samples provided pre- and post-BCG vaccination from 10 individuals to identify specific receptors and TCR clones that are induced by BCG. Overall, there was no change in the diversity of gTCR or dTCR clonotypes in post- vs pre-BCG samples. Furthermore, the frequencies of TCR variable and joining region genes were minimally modulated by BCG vaccination at either the gTCR or dTCR loci. However, the gTCR and dTCR repertoires of individuals were highly dynamic; a median of ~1% of gTCR and ~6% of dTCR in the repertoire were found to significantly expand or contract in post- vs pre-BCG comparisons (FDR-q Data was collected with consent of the participants as part of protocols approved by local IRBs (see manuscript for details)