αvβ6/αvβ1 integrin inhibitor bexotegrast targets pathologic cells in fibrotic human lung tissue

Summary: This dataset includes single-nuclei RNA-sequencing (snRNA-seq) data obtained from fibrotic human precision-cut lung slices (PCLS) that was generated to support the publication of the article, “αvβ6/αvβ1 integrin inhibitor bexotegrast targets pathologic cells in fibrotic human lung tissue”. This study was designed to assess the pharmacodynamics of the compound bexotegrast, a dual inhibitor of TGF-b-activating integrins, in inhibiting targeted TGF-b signaling in pathologic cells of the fibrotic lung. Files: avb6_avb1_integrin_inhibitor_bexotegrast_publication.rds: merged samples from n = 19 human PCLS samples (112,979 total nuclei) as a Seurat object. avb6_avb1_integrin_inhibitor_bexotegrast_publication.tsv: metadata with sample treatment type (vehicle, bexotegrast, ALK5i) and cell type annotation. Methods: Library preparation and sequencing technology: 10X Genomics 3’ RT Chip M, Illumina NovaSeq S4. Data analysis: Seurat 4.3.0 was used for all processing and analyses. Nuclei with less than 500 genes and more than 4000 genes and with 10% or greater mtDNA content were filtered out. Genes not present in at least 3 nuclei were filtered out. Samples were merged and data was normalized and scaled. PCA was performed followed by batch reduction using Harmony 1.2.0. Clusters were identified using FindClusters and a projection of the nuclei was generated with RunUMAP.