Long-term culture with charcoal-stripped serum effect on the androgen-dependent LNCaP prostate cancer cell line. Long-term culture with charcoal-stripped serum effect on the androgen-dependent LNCaP prostate cancer cell line

To further determine the gene expression in the castration resistant condition for treating PCa, we generated and characterized an androgen-independent LNCaP-AI cell line by long-term culture of androgen-dependent LNCaP cells in RPMI-1640 medium containing charcoal-stripped serum. Overall design: Three independent experiments were performed using three independent samples for LNCaP and LNCaP-AI cell lines.