Lipid profile of RAW264.7 cells treated with or without LPS + IFNγ for 24 h.

RAW264 mouse leukemic cells (2.5x105 per well) were added to 6 well-plates and incubated with either vehicle (“None”) or LPS (0.1 μg/mL well) + INF-γ (100 U/mL) for 24 h. at 37°C prior to incubation for 30 min with either vehicle, URB597 (1 μM), flurbiprofen (10 μM), URB597 + flurbiprofen or (R)-Flu-AM1 (10 μM). The samples were assayed in two batches. In order to minimise effects of inter-batch variations, which were seen for some of the lipids, the median value for each lipid and batch under vehicle-treated “None” conditions were set to unity and all other values for the batch were expressed relative to these median values. Data are given as medians, n = 5–6, with the range in brackets, and the statistical test used was a two-way robust Wilcoxon analysis [25]. L: main effect LPS treatment, I: main effect inhibitor treatment, T x I, interaction term. Note that since there are multiple analysis, a case can be made that the Bonferroni correction should be used. Significance levels below 0.0025 (= 0.05/20, i.e. Bonferroni-corrected) are shown in bold.Effects of FAAH and/or COX inhibitors.