STAT1 (Interferon-alpha) ChIP-ChIP from HeLaS3 Cells using the NimbleGen ENCODE Array

STAT1 ChIP DNA was isolated from HeLaS3 cells following Interferon-alpha stimulation for 30 min and from HeLaS3 cells left untreated. These two STAT1 ChIP DNA samples were combined and compared in the ENCODE regions on NimbleGen ENCODE arrays. Keywords: ChIP-chip Cultures of HeLaS3 cells were divided in half and were either stimulated with Interferon-alpha for 30 min. or left untreated. STAT1-ChIP DNA was isolated from both halves of the culture, labeled and then combined and hybridized to a NimbleGen ENCODE tiling array that contains overlapping 50nt oligonucleotide probes. The series is comprised of three biological replicates, hybridized separately to three arrays.