Transcriptomic profiling of microglia and astrocytes throughout aging

Purpose:The goal of this study are to understand the role of miceoglia and astrocyte in both physiological and pathological conditions. Methods: Microglia and astrocyte were isolated from 5 time points WT/APP-PS1 mice in triplicate. mRNA were generated by SMART seq2 using Illumina Novaseq platform. Conclusions: Using DESeq2 to sequence polyA-selected mRNAs from microglia and astrocytes isolated from whole brain. We mapped >85% of reads for all of our sample. We found good reproducibility between the replicates Overall design: We performed RNA-seq in microglia and astrocytes freshly isolated from Wild-type(WT)/APP-PS1(AD) mice brains across the lifespan to analyze the longitudinal genes expression profile.