Parallelism in gene expression between foothill and alpine ecotypes in Arabidopsis arenosa

We investigated differences in gene expression between pairs of foothill and alpine populations across four mountain regions and tested whether foothill and alpine populations responded in a similar way to environmental changes. We raised Arabidopsis arenosa plants in growth chambers under different treatments that varied in temperature and irradiance. When plants were at the 14-leaf stage, we collected one leaf per plant for RNA-seq analysis (96 individuals sequenced).