Additional file 1 of Integrative analysis of cell adhesion molecules in glioblastoma identified prostaglandin F2 receptor inhibitor (PTGFRN) as an essential gene

Additional file 1. Figure S1. Flow chart describing the use of various datasets to identify the importance of CAMs for glioma development and progression. CAMs (n = 518) derived from various sources that included CAMs family genes based on protein domain structures such as cadherin, integrin, and immunoglobulin and Gene Ontology (GO) terms related to cell adhesion and ‘CAMs and Homo sapiens’ keyword query against NCBI Entrez annotations and various literature, manually curated and compiled and were used in this study. The first and second branch depicts the use of TCGA Agilent dataset to identify differentially expressed CAMs in GBM followed by the possible causes of their differential regulation and survival analysis, respectively. The last branch identifies the GSC-specific CAMs over both NSCs and DGCs. Functional studies were carried out for PTGFRN which is deregulated in GBM and GSCs and indicates a poor prognosis. The number in brackets shows the number of samples used for analysis. GSCs: Glioma-like stem cells, NSCs: Normal neural stem cells, DGCs: Differentiated glioma stem cells, CNV: Copy number variation.