Oligomerization of Cry1Ac in the presence of BBMV from resistant and susceptible larvae of <i>P</i>. <i>gossypiella</i>.

Cry1Ac activated toxin (0.5 μg) was incubated with BBMV (15 μg) from P. gossypiella susceptible APHIS-S (lane 5), resistant AZP-R (lane 6) separated by SDS-PAGE after three min heating at 50°C and revealed in western blot using anti-Cry1Ac antibody. Lane 1, shows MW markers; lane 2, Cry1Ac activated toxin; lane 3, BBMV from APHIS-S; lane 4, BBMV from AZP-R. Numbers under the 200 kDa bands represent the percentage of pixels relative to the 200 kDa band of APHIS-S BBMV with Cry1Ac toxin that corresponds to 100, calculated after scanning densitometry of the bands.