siRNA knockdown of neonatal rat cardiac myocytes and fibroblasts

Primary neonatal rat cardiac myocytes or fibroblasts were isolated and subjected to siRNA mediated Yap knockdown Primary cardiac myocytes and fibroblasts were isolated from two-day old rats via neonatal heart dissociation kit (Miltenyi Biotec) according to the manufacturer’s protocol followed by percoll gradient separation. Cells were transfected with siRNAs designed against Yap, or universal negative control siRNAs. Transfected cells were collected 48 hours post transfection for RNAseq.