Ginger inhibits the invasion of ovarian cancer cells SKOV3 through CLDN7, CLDN11 and CD274 m6A methylation modifications

Background: Ginger is a customary aromatic vegetable imbued with multifarious functional active ingredients and bears considerable medicinal and alimentary properties. Numerous studies have shown that ginger and its active ingredients exhibit suppressive effects on manifold tumours, including ovarian cancer (OC). Nevertheless, the molecular mechanism by which ginger inhibits OC is not clear. The objective of this research was to look into the function and mechanism of ginger in OC.Methods: The estimation of n6-methyladenosine (m6A) levels was accomplished through the employment of the m6A RNA methylation quantification kit, and RT-qPCR was utilized to determine the expression of m6A-related genes and proteins. Through the scratch assay and Transwell assay, the impact of ginger on SKOV3 migration in ovarian cancer cells was investigated. The m6A methylationome was detected by MeRIP-seq, following analysis of the data. Differential methylation of genes was assessed utilizing RT-qPCR and western blotting.Results: Ginger significantly inhibited the migration of SKOV3 and curtailed m6A levels in OC cells. The 3'UTR region is the major site of modification for m6A methylation, and its key molecular activities include Cell Adhesion Molecules, according to meRIP-seq results. Moreover, it was observed that Ginger aids significantly in downregulating the CLDN7, CLDN11 mRNA, and protein expression.Conclusions: Ginger inhibits ovarian cancer cells' SKOV3 invasion by regulating m6A methylation through CLDN7, CLDN11, and CD274.