PKC responsive sites of RIP140 and their consensus motif-specific chaperones.

Twelve phosphorylation sites of RIP140 purified from insect cell cultures have been identified by LC-ESI-MS/MS analysis. Ser-102 in N-terminal and Ser-1003 in C-terminal are consensus and responsive to PKC and 14-3-3 binding. aSequences adjoining to the residues were compared to consensus motifs of known kinases/chaperone. bPKC: S/T-X-K/R, K/R-X-X-S/T, K/R-X-S/T; 14-3-3: R(S)X1,2pSX(P), RXX(pS/pT)XP and RXFX(pS/pT)XP where S, T, K, R, and P are single-letter codes of amino acids, and X can be any amino acid. Small case p refers to phosphorylation at respective residue.