Chromatin-associated Microprocessor assembly is regulated by PRP40 – U1 snRNP auxiliary protein

In order to assess the possible effects of prp40ab double mutant on co-transcriptional miRNA biogenesis, small RNA sequencing was performed as well as RNA PolII and DCL1 distribution were tested on MIR genes (ChIPseq). Examination of miRNA biogenesis and RNA PolII, DCL1 distribution in prp40ab mutant