Genome analysis of Fisheries organisms (KAP220263)

Overall Goal: To be able to use intangible information as a high value-added industrial material by decoding, analyzing, and processing large-capacity dielectric/transcriptional/functional gene/genetic marker information produced from 13 kinds of aquatic organisms. Completed the map of 13 types of standard genomes of major aquatic organisms (construction of 3 or more types of standard genomes) Development of useful genes and genetic markers related to major economic traits Building a foundation for utilizing genetic databases and big data specialized in aquatic life Discovering highly functional biomaterials derived from big data of marine life genomes Investigation of the function of four useful genes of aquatic organisms in the second year and production of information on the genomes of new aquatic organisms. -Discovery and functional study of useful genes of pollock, gangdo bridge, white copperfish, and large-billed mudskipper. Analysis of genomes and transcription bodies such as loach, horse ratfish, red fly, mackerel, eel, catfish, etc.. Fish microbiome analysis. Analysis and processing of large-capacity omix information. Analysis of genomes and transcription of species subject to detoxification of genomes in the second year ( loach, horsefish, mackerel, eel, catfish, etc.). Genetic decoding method and scope for the second year: Based on the dielectric size of 1.0 Gb. Isomorphic transcription production for Annotation: Field expression gene analysis using IsoSeq: Brain, gill, soy sauce, kidney, spleen, gastrointestinal tract, rhyming water, intestines, muscles, shells, blood cells, reproductive RNA after pooling in the same amount. Comparative analysis of near-related genomes: Misguraus anguillicaudatus, a similar species, and Paramisg (urnus dabryanus) comparative genomes. Microbiome Analysis: 16SrRNA Sequencing and Fungal Analysis of Surface and Intestinal Microorganisms, Comparative Analysis of Beneficial and Pathogenic Genes, Functional, Blood Indicators, Intestinal Microbiological Changes and Health Functional Analysis using Mouse. Genetic Functional Analysis: Comparative analysis of expression patterns by tissue and condition of water temperature or immune-related genes, and functional analysis through recombinant protein or peptide synthesis -Discovery of genomic information-based biomarkers and biomaterials: Simple Sequence Repeat extraction based on genomic and transcription sequences, MS Marker development for species and collective analysis, and candidate peptide sequences (pI, net charge, secondary structure, etc.) -Construction of chromosome-level standard genomes: [Mackerel] Production of chromosome-level data from the International Spinal Animal Genome Project (VGP) 1) technical aspect. World leader in comparative genetics: It is expected to lead the field of comparative genetics through the development of innovative comparative genomic analysis tools that are tailored to the new 3rd generation standard genomic format and provide new research opportunities for structural variation analysis.. Securing the 3rd generation standard gene construction pipeline: By first attempting to build the 3rd generation standard gene at the level of chromosomes that are not yet present in Korea, the pipeline is constructed and secured to provide the foundation for future research.. The foundation for subsequent multi-omix research: Establishing high-quality standard genes will pave the way for future researchers at home and abroad for integrated research from a multi-omix perspective such as transcription, metabolism, and epigenetc. 2) economic and industrial aspects. Diversification of farmed fish species and molecular breeding: It is expected to be used for breeding in the future by breaking away from the current fishing practices that focus only on some fish species and securing genetic markers and genetic information for more diverse species. -Discovery and utilization of new genetic resources of marine life: Finding useful genetic resources such as antibacterial peptides in various marine life and seeking specific ways to utilize them. -Preparation for sharing profits of genetic resources: According to the Nagoya Protocol Digital Sequence Information (DSI), the standard genetic information produced in this project will be used as an advantage for Korea in the global genetic resource competition. 3) social aspect. Transmission of genetic resources for future generations: By constructing genetic information of domestic endemic and endangered species in a chromosome-level standard genetic form, high-quality genetic information about the species is recorded and transmitted to future generations.. Basic study for biodiversity conservation: Based on the understanding of specific genetic characteristics of each species, it is possible to understand evolutionary adaptation mechanisms and ecological adaptation in depth and use them for future biodiversity conservation.