Prevalence of culicine salivary antibodies in non-human primates living in national parks in Thailand

Macaques are widely distributed in Thailand with remarkable adaptation to living close to humans in residential, religious sites, markets, and tourist areas. They play an essential role in the persistence of pathogens in the environment. As reservoir hosts, they are exposed to hematophagous vectors that secrete saliva, a cocktail of bioactive molecules including antigenic components stimulating host antibody production. Subsequent to the detection of mosquito-borne pathogens in macaques living in national parks, we aimed to determine the seroprevalence of antibodies to crude salivary gland extracts (SGE) from culicine mosquitoes (Aedes aegypti [Linnaeus, 1762], Ae. albopictus [Skuse, 1895], and Culex quinquefasciatus [Say, 1823]) and compare individual titers between macaque species/national parks (33, Macaca arctoides [I.Geoffroy Saint-Hilaire, 1831] [Primates: Cercopithecidae] from Kaeng Krachan, 23 M. leonina leonina [Blyth, 1863] [Primates: Cercopithecidae] from Khao Yai, and 4 M. f..., All data sets of antibody titers of macaque bloods, mosquito salivary grand extraction protein profiles, and PRNT titers of virus detections were recored in Microsolf excel spreed sheet solfware, USA., , # Dataset-Med Vet Entomo

[https://doi.org/10.5061/dryad.tht76hf7z](https://doi.org/10.5061/dryad.tht76hf7z)

## Description of the data and file structure

Figure1 file: data set Map of serum samples collected from macaques in Thailand national parks from 2018 to 2019.

Fig2 file: Immunoglobulin G SGE (salivary gland extract) antibody endpoint titers (log2) to three mosquito species: *Aedes aegypti*(circle), *Ae. albopictus*(square), and *Culex quinquefasciatus*(triangle) from national park-dwelling macaques determined by ELISA. Serum samples were collected from *M. leonina, M. arctoides,* and *M. fascicularis* from Khao Yai (KY) National Park in Nakhon Ratchasima, Kaeng Krachan (KK) National Park in Prachuap Khiri Khan, and Mu Ko Ranong (RN) National Park in Ranong, respectively.

Fig3 file: Salivary gland extract antibody titers (log2) against *Aedes aegypti*(A) and *Ae. albopictus*(B) between dengue virus/DENV- (A) and Zika virus/ZIKV- (B) positive (+ve) and -negative (-ve) stump-ta...,