Nascent transcript analysis of glucocorticoid crosstalk with TNF in Beas-2B airway epithelial cells

This study addressed mechanisms through which glucocorticoids exert repressive effects on pro-inflammatory TNF signaling using Global Run-on Sequencing (GRO-seq) in Beas-2B airway epithelial cells treated with dexamethasone and/or TNF for 10 or 30 minutes. Overall design: Duplicate samples for each of the treatment conditions [vehicle (ethanol), TNF (20 ng/ml), dexamethasone (100 nM) or TNF+dex] at each time point (10 or 30 minutes) were sequenced and included in the analyses..