FG repeats drive co-clustering of nuclear pores and P. granules in the C elegans germline [RNA-seq]

Condensates that accumulate small RNA biogenesis factors (nuage) are common in germ cells and often associate with nuclei. In the C. elegans germline, P granules overlay large clusters of nuclear pores and this organization has been proposed to facilitate surveillance of nascent transcripts by Argonaute proteins enriched in P granules. We report that co-clustering of nuclear pores and P granules depends on FG repeat containing nucleoporins and FG repeats in the Vasa class helicase GLH-1. Mutants that prevent co-clustering are fertile under standard growth conditions and only mis-regulate a minority of genes, including replication-dependent histones. Our observations suggest that association with nuclear pores, while non-essential for genome surveillance, may serve to tune mRNA flow through P granules and other nuage condensates. Overall design: ribo-depleted total RNA sequencing of adult N2 and npp-214? mip? mutants