Mutational Genomics to identifiy candidate genes in oat. MutSeq_Oat

We resequenced independent mutants and used that data to identify candidate genes responsible for a distinct phenotype.Our method was tested by re-cloning the gene SAD3 using four mutants that exhibit loss of the associated phenotype.After that proof of concept, we aplied the same method, again with four mutants, to identify a candidate for PAL2 (SAD4-like).