Data for: Sensitive and specific detection of tumor-derived exosomes using nanopore-crystal microchips

Tumor-derived exosomes (tExos) have emerged as promising circulating biomarkers for early cancer diagnosis. However, the sensitivity and specificity of existing assays often limit the clinical translation of tExos. In this study, we present a highly versatile microfluidic platform, termed the nanopore-crystal microchip (NC-Chip), for specific isolation and ultrasensitive detection of tExos in as little as 0.5 μL of plasma samples from pancreatic cancer (PC) patients. The NC-Chip incorporates a herringbone-patterned hierarchical porous hydrogel scaffold, enabling fluid manipulation, size exclusion, immunoaffinity capture, and signal amplification. These integrated features significantly enhance the sensitivity and specificity of tExos assays in complex clinical scenarios. Utilizing an eight-protein signature, the NC-Chip can distinguish patients with pancreatitis and non-metastatic PC with 100% accuracy in the training cohort and 94.9% accuracy in the validation cohort. This platform is ..., The mean, SD, and LOD were computed utilizing established standard formulas. A student’s t-test with two tails was employed to assess significance. The intensities of individual protein markers measured by the NC-Chip were normalized using Min-Max. The normalized intensities of eight protein markers were weighted together to create the PC signature by LDA. The nonparametric, two-tailed Mann-Whitney U test for binary classification was used to determine P values for pairwise comparisons. With a post-hoc Dunn’s test for pairwise multiple comparisons, a Kruskal-Wallis one-way ANOVA was used to determine the overall and group pair P values for ternary classification. To evaluate the AUC, sensitivity, specificity, and accuracy of PC diagnosis, ROC analyses were developed for individual markers or marker combinations. The discriminant function model was initially constructed using data from the training cohort, and then it was applied to classify patients in the validation cohort. The validat..., , # README:Sensitive and specific detection of plasma tumor-derived exosomes using nanopore-crystal microchips

Description of the data and file structure
(1) Fig.3e-Fluorescence_enhancement_factor.csv
This document is the enhancement factor of fluorescent FDG, Cyanine3, and Tex-red inside Flat-Chip, Solid-Chip, and NC-Chip. Before use, the FDG solution at FDG: β-Gal ratio of 1:1 was created and reacted for 30 min at 37 °C in the dark. The enhancement factor was calculated by ∆E,exp = (IE−Ix)/(I0−Ix), where IE represents the intensity on the NC-Chip or Solid-Chip on the herringbone pattern, I0 represents the intensity on the Flat-Chip channel, and Ix represents the background correction. The FDG represents fluorescein-di-β-d-galactopyranoside. The β-Gal represents β-galactosidase. The NC-Chip represents nanopore-crystal microchip. The Solid-Chip represents solid herringbone chip. The Flat-Chip represents an assay chip on clean glass slides.

(2) Fig.3f-Simulated_reflection_spectra.csv
Th...